UFC officials announced the match-up on Tuesday.
Gestelum, currently coaching The Ultimate Fighter: Latin America 2, is coming off his first career loss. The move back to the middleweight division comes at the behest of UFC officials after the 23-year-old failed to make weight in two of his last three welterweight bouts.
Marquardt returned to the 185-pound weight class in 2014 after spending two years competing at a welterweight. The former Strikeforce champion has gone 1-1 since returning to the middleweight division. He is coming off a loss to Brad Tavares in his last outing on Jan. 3.